Saliva biomonitoring using LPME-GC/MS method to assess
In the present study, an alternative method for the determination of bisphenol A (BPA) and phthalate metabolites in saliva was developed and validated using hollow fiber liquid phase microextraction (HF-LPME) for sample preparation and gas chromatography coupled to ion trap mass spectrometry (GC/MS) for analysis.
Saliva biomonitoring using LPME-GC/MS method to assess
Bisphenol A and phthalates are endocrine disruptors widely used as chemical additives mainly in plastic products, including materials for dentistry procedures. Besides, many plasticizers have been associated with important diseases requiring performed methods for their quantification. In the present study, an alternative method for the determination of bisphenol A (BPA) and phthalate
Saliva biomonitoring using LPME-GC/MS method to assess
In the present study, an alternative method for the determination of bisphenol A (BPA) and phthalate metabolites in saliva was developed and validated using hollow fiber liquid phase...
Effect of the nature and concentration of phthalates
suitability of existing in vitro methods for the determination of the release of phthalates 2) investigate the effect of the nature of the phthalate on their release in saliva simulant under foreseen human conditions, and 3) investigate the effect of the percentage of different phthalates in PVC materials on their release in saliva simulant.
Levels of phthalates and bisphenol in toys from Brazilian
The synthetic saliva was added in two consecutive portions to simulate real-life conditions since the release of saliva into the mouth occurs as a function of time. The contents of tube B were subjected to the extraction procedure using 1.0 mL of hexane for PAEs and 1.0 mL of dichloromethane for BPs.
- Which phthalate is detected in GC-MS?
- At least one PAE was detected in 81% of samples, and di-n-octyl phthalate was found in higher concentration, achieving the migration of almost 30 μg per g of sample. GC-MS; Pa-DPX; Parallel-disposable pipette extraction; Phthalate migration; Saliva simulant. Copyright © 2020 Elsevier B.V. All rights reserved.
- What are phthalic acid esters?
- Phthalic acid esters (“phthalates”) are the most commonly used plasticizers in polyvinyl chloride (PVC) based products due to their compatibility and softening capability . The plasticiser content can be up to 50% by weight.
- How accurate are phthalate ion abundance ratios?
- Replicate measurements (n=15) on the ion abundance ratios for each phthalate was determined to calculate the precision of the calibration, and the relative standard deviations of the qualifier to the quantifying ion. The relative standard deviations on the relative abundances did not exceed 10% for all the phthalates tested.
- Which phthalates were not tested?
- The remaining three phthalates from the EU Scientific Committee for Toxicity, Ecotoxicity and the Environment (CSTEE) list—dibutyl phthalate (DBP), benzyl butyl phthalate (BBP) and di-n-octyl phthalate (DnOP)—were not tested due to the unavailability of suitable materials. Previousarticlein issue Nextarticlein issue Keywords Agitation methods
- Does PAE migrate from 21 samples?
- The Pa-DPX-GC-MS method was successfully applied to determine the migration of PAE from 21 samples. At least one PAE was detected in 81% of samples, and di-n-octyl phthalate was found in higher concentration, achieving the migration of almost 30 μg per g of sample.
- What is the molecular ion peak for phthalate compounds with long chain alkyl groups?
- The molecular ion peak for phthalate compounds with long chain alkyl groups is usually weak and not always present in the mass spectra, but the (M–R)+and (M–OR)+(R=alkyl group) fragments can be a secondary form of identification.
