Urinary Concentrations of Major Phthalate and Alternative
Urinary Concentrations of Major Phthalate and Alternative Plasticizer Metabolites in Children of Thailand, Indonesia, and Saudi Arabia, and Associated Risks Urinary Concentrations of Major Phthalate and Alternative Plasticizer Metabolites in Children of Thailand, Indonesia, and Saudi Arabia, and Associated Risks
Determination of human urinary metabolites of the plasticizer
Determination of human urinary metabolites of the plasticizer di(2-ethylhexyl) adipate (DEHA) by online-SPE-HPLC-MS/MS Di(2-ethylhexyl) adipate (DEHA) is a plasticizer and phthalate substitute used in various consumer products. Relevant population exposures have to be assumed.
Determination of human urinary metabolites of the plasticizer
Di(2-ethylhexyl) adipate (DEHA) is a plasticizer and phthalate substitute used in various consumer products. Relevant population exposures have to be assumed. In this study we describe the determination of three specific side chain-oxidized monoester metabolites of DEHA, mono-2-ethyl-5-hydroxyhexyl adipate (5OH-MEHA), mono-2-ethyl-5-oxohexyl adipate (5oxo-MEHA), and mono-5-carboxy-2
Determination of human urinary metabolites of the plasticizer
Overall, our detection of plasticizers in foods from U.S. chains is consistent with recent biomonitoring studies that have detected urinary metabolites of DEHA, DEHT, and DINCH in various...
Urinary Concentrations of Major Phthalate and Alternative
Urinary Concentrations of Major Phthalate and Alternative Plasticizer Metabolites in Children of Thailand, Indonesia, and Saudi Arabia, and Associated Risks Inae Lee Inae Lee School of Public Health, Seoul National University, Seoul 08826, Republic of Korea More by Inae Lee https://orcid.org/0000-0002-8031-2105 Claudia Plmke Claudia Plmke
- How do we measure oxidized monoester metabolites of DEHTP in human urine?
- We have developed a fast and reliable quantification method for specific side chain oxidized monoester metabolites of DEHTP in human urine using authentic analytical standards and isotope labelled internal standards. In a pilot biomonitoring study we provide first human data on internal exposure to DEHTP.
- Which side chain oxidized monoester metabolites are a biomarker of DEHA exposure?
- In this study we describe the determination of three specific side chain-oxidized monoester metabolites of DEHA, mono-2-ethyl-5-hydroxyhexyl adipate (5OH-MEHA), mono-2-ethyl-5-oxohexyl adipate (5oxo-MEHA), and mono-5-carboxy-2-ethylpentyl adipate (5cx-MEPA) in human urine as potential biomarkers of DEHA exposure.
- How are phthalates and alternative plasticizers metabolites measured?
- Metabolites of major phthalates and alternative plasticizers were measured in the urine samples by HPLC-MS/MS. Urinary metabolite levels differed substantially between the three countries.
- Which metabolites can be used as biomarkers of DEHA exposure?
- In the quest for metabolites to be used as specific biomarkers of DEHA exposure Silva et al. tentatively identified two specific 2-ethylhexyl side chain-oxidized monoester metabolites in vitro with human liver microsomes: mono-2-ethylhydroxyhexyl adipate (OH-MEHA) and mono-2-ethyloxohexyl adipate (oxo-MEHA).
- Which carboxylated metabolite can be detected and quantified in urine?
- The carboxylated metabolite 5cx-MEPTP could be detected and quantified in 32 of the 34 spot urine samples. The second most abundant metabolite was 5oxo-MEHTP with a detection rate of 21%. Detection rates for the other metabolites were lower (detailed results given in Table 5).
- Can MHS-SPME-GC-MS be used to analyze two plasticizer metabolites?
- Validation of MHS-SPME-GC-MS method for the analysis of two In the present work, an optimization and validation of an analytical method for the determination of two plasticizer metabolites, 2-ethylhexanol and 4-heptanone in urine, were carried out by multiple headspace solid-phase microextraction (MHS-SPME) coupled to GC–MS.
