Hindbrain defects induced by Di-butyl phthalate (DBP)
More specifically, DBP is noted to induce cardiac malformation, pericardial edema and altered heart rates (Pu et al., 2025; Sun and Li, 2025), skeletal and spinal issues (Pu et al., 2025; Qian et al., 2025), craniofacial and jaw defects (Jergensen et al., 2025) and most recently defects in eye development (Barbagallo et al., 2025).
Di-butyl phthalate (DBP) induces defects during embryonic eye
We find defects in eye development at 96 h post fertilization including a decrease in the size of the lens and retina in DBP-treated embryos. Defects in eye vascularization as well as loss of the optic nerve and optic tectum were also noted.
Di-butyl phthalate (DBP) induces defects during embryonic eye
DBP induces vascular defects in eye development Vascularization of the eye at 96 hpf was visualized with the Tg (fli1:EGFP) transgenic embryos where GFP is expressed in the developing endothelial blood vessels of the eye (Lawson and Weinstein 2002 ).
Di-butyl phthalate (DBP) induces craniofacial defects during
DBP induces a reduction in craniofacial development. a DBP treatment caused a significant reduction in all measurements except the intraocular distance which was increased in DBP treated embryos. Trial 3, n per trial: 10, total n: 30. Student’s T test was performed, asterisk denotes p < 0.05 and bars note standard deviation.
Di-Butyl Phthalate (DBP) Induces Defects in Eye Development
Di-Butyl Phthalate (DBP) Induces Defects in Eye Development in Zebrafish Mentor/s Nicole Roy Participation Type Poster Abstract Di-butyl phthalate (DBP) is commonly added to make plastics softer and more pliable and is found in a variety of consumer and industrial products.
- Does di-butyl phthalate induce defects during embryonic eye development in zebrafish?
- Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Barbagallo, S., Baldauf, C., Orosco, E. et al. Di-butyl phthalate (DBP) induces defects during embryonic eye development in zebrafish.
- What is di-butyl phthalate (DBP)?
- Di-butyl phthalate (DBP) is a phthalate ester (PAEs) added during the manufacturing of plastics to make them stronger, yet more pliable. DBP is noncovalently bound to plastics resulting in leaching into the environment.
- Does developmental toxicity of DBP affect early embryonic eye?
- Here, we utilized the zebrafish model and assess the developmental toxicity of DBP on the early embryonic eye. Specifically, we treat embryos with DBP during the gastrula stage when embryonic patterns that underlie adult structures are established. We find decreases in the size of the lens and retina.
- Does DBP decrease the size of the lens and retina?
- DBP decreases the size of the lens and retina. Live images of control ( A ), vehicle ( B) and DBP ( C) treated embryos at 96 hpf. Control and vehicle demonstrate normal size lens and retina. Lens is noted as dashed line, retina as solid line.
- What are defects in eye development at 96 h post fertilization?
- We find defects in eye development at 96 h post fertilization including a decrease in the size of the lens and retina in DBP-treated embryos. Defects in eye vascularization as well as loss of the optic nerve and optic tectum were also noted.
- Can phthalates cause developmental toxicity?
- The developmental toxicity of phthalates has been increasingly studied in the past years, and progress has been made on the effects of DBP on some tissues. Recent studies on DBP have noted skeletal defects, spinal curvatures and abnormal movement (Pu et al. 2020; Qian et al. 2020) (0.2 µg/L, 250 µg/L).
