Special stains in cytology - SlideShare

                                               
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  • Is DPX a good alternative to xylene?
  • With DPX new, Merck now provides an alternative for all users who want to continue working with xylene, although Neo-Clear® and the associated mounting medium Neo-Mount™ already offer an alternative option with significantly lower risks for users.
  • Why is DPX a plasticizer?
  • It preserves the stain and dries quickly. A mixture of distyrene (a polystyrene), a plasticizer (tricresyl phosphate), and xylene, called DPX, was introduced in 1939 and later modified by the substitution of a more satisfactory plasticizer, dibutylphthalate (butyl, phthalate, styrene - BPS).
  • What is xylene used for?
  • It is characterized by high evaporation rates, resulting in the typical laboratory odor. Xylene is often used in the dehydration steps during histoprocessing and prior mounting. In combination with xylene, water-free mounting media, such as Entellan® New and DPX, should be used for permanent mounting and long-time storage of samples.
  • Is xylene a dangerous solvent?
  • These can cause health problems, as they may, for example, be carcinogenic or teratogenic. Merck has reduced this risk with its new water-free mounting medium DPX new. Xylene is a particularly hazardous solvent in laboratories. It is characterized by high evaporation rates, resulting in the typical laboratory odor.