Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA PLoS One. 2017 Sep 11;12 (9):e0184507. doi: 10.1371/journal.pone.0184507. eCollection 2017. Authors
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA Konstantin A. Blagodatskikh, Vladimir M. Kramarov, Ekaterina V. Barsova, Alexey V. Garkovenko, Dmitriy S. Shcherbo, Andrew A. Shelenkov, Vera V. Ustinova, Maria R. Tokarenko, Simon C. Baker, Tatiana V. Kramarova,
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA. Sign in | Create an account. https
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA Authors: Konstantin A. Blagodatskikh Pirogov Russian National...
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method
RESEARCH ARTICLE Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA Konstantin A. Blagodatskikh1, Vladimir M. Kramarov1,2
- What is iDOP-PCR?
- iDOP-PCR (Improved DOP-PCR) is a modified version of the classic DOP-PCR method. It uses a new thermostable DNA polymerase (SD polymerase) with strong strand-displacement activity and adjustments in primers design.
- Is DOP-PCR a good method for WGA?
- While DOP-PCR was once considered a pioneering method for WGA, it only provides low genome coverage and a high allele dropout rate compared to more modern techniques. To address these limitations, we describe an improved DOP-PCR method called iDOP-PCR.
- Why is IDOP-PCR better than other WGA methods?
- In summary, iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an input material.
- What is DOP-PCR?
- One example of a WGA method is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple, fast and inexpensive WGA technique. There are a number of WGA methods that have been developed over the years.
- Does IDOP-PCR improve the quality of amplified DNA libraries?
- iDOP-PCR provided a better quality of the amplified DNA libraries compared to other whole genome amplification (WGA) methods tested, especially when low amounts of genomic DNA were used as an input material. The amplified DNA libraries were evaluated by analysis of short tandem repeat genotypes and NGS data.
- How does iDOP-PCR perform with low amounts of genomic DNA?
- iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an input material. The amplified DNA libraries were evaluated by analysis of short tandem repeat genotypes and NGS data.
