In vitro cytotoxic effects of DEHP-alternative plasticizers
DEHP, ATBC and DINCH are considered as potentially toxic in the standard EN 10993-5. All plasticizers primaries metabolites cause a decrease in cell viability except MOTM. MEHT, MINP, MINCH reduce significantly the cell proliferation at 0.1 mg/ml. MINCH causes a very high inhibition of cell proliferation.
In vitro cytotoxic effects of DEHP-alternative plasticizers
In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices. They carry a high exposure risk for both humans and the environment in clinical situations.
In vitro cytotoxic effects of secondary metabolites of DEHP
Objectives: Our study focuses on the in vitro cytotoxicity of two alternative plasticizers (DINCH and DINP) contained in certain medical devices. They are likely to migrate and be transformed in vivo into the primary and secondary metabolites by a metabolism similar to that of DEHP.
In vitro cytotoxic effects of secondary metabolites of DEHP
Two alternative plasticizers (diisononyl phthalate (DINP) and 1,2-cyclohexane dicarboxylic acid (DINCH)) have been considered by manufacturers as a replacement for DEHP in many PVC products, such as medical devices and food packaging, but are limited in toys with concentrations inferior to 0.1% by weight of the plasticized material (European
Alternative Plasticizers As Emerging Global Environmental and
In Vitro Cytotoxic Effects of DEHP-Alternative Plasticizers and Their Primary Metabolites on a L929 Cell Line. Chemosphere 2017 , 173 , 452 – 459 , DOI: 10.1016/j.chemosphere.2017.01.026 [ Crossref ], [ PubMed ], [ CAS ], Google Scholar
- Are DEHP-alternative plasticizers cytotoxic?
- In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices. They carry a high exposure risk for both humans and the environment in clinical situations.
- Are alternative plasticizers cytotoxic in vivo?
- Objectives: Our study focuses on the in vitro cytotoxicity of two alternative plasticizers (DINCH and DINP) contained in certain medical devices. They are likely to migrate and be transformed in vivo into the primary and secondary metabolites by a metabolism similar to that of DEHP.
- Is Deha cytotoxic?
- DEHA showed no cytotoxicity, but its corresponding monoester (MEHA) produced a cytotoxic effect at 0.05 mg/ml. In clinical situations, medical devices can release plasticizers, which can come into contact with patients. In vivo, the plasticizers are quickly transformed into primary metabolites.
- Are plasticizers cytotoxic to PVC medical devices?
- We studied the cytotoxicity of plasticizers added to PVC medical devices using the MTT test, and the cell line (L929 cell line) described in EN 10 993-5. The concentrations studied were those found in biological fluids during direct contact with the medical devices (Takahashi et al., 2008, Kambia et al., 2011; Scenirh, 2015; Eckert et al., 2016).
- Which plasticizers are toxic?
- DEHP, ATBC and DINCH are considered as potentially toxic in the standard EN 10993-5. All plasticizers primaries metabolites cause a decrease in cell viability except MOTM. MINCH causes a very high inhibition of cell proliferation. Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices.
- Are plasticizers biocompatible with medical devices?
- Cytotoxicity assays were performed on L929 murine cells according to the ISO-EN 10993-5 standard design for the biocompatibility of medical devices. The tested concentrations of plasticizers (0.01, 0.05 and 0.1 mg/ml) covered the range likely to be found in biological fluids coming into direct contact with the medical devices.
